VPS25 Antibody | CSB-PA887108LA01HU

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CSB-PA887108LA01HU
Availability:
3 to 7 Working Days
  • VPS25 Antibody
  • Western blot<br />
 All lanes: VPS25 antibody at 3µg/ml<br />
 Lane 1: Mouse kidney tissue<br />
 Lane 2: HL60 whole cell lysate<br />
 Lane 3: THP-1 whole cell lysate<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/10000 dilution<br />
 Predicted band size: 21 kDa<br />
 Observed band size: 21 kDa<br />
  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA887108LA01HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA887108LA01HU at dilution of 1:100
  • Immunofluorescence staining of Hela cells with CSB-PA887108LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

VPS25 Antibody | CSB-PA887108LA01HU | Cusabio

VPS25 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: VPS25

Aliases: Vacuolar protein-sorting-associated protein 25 (hVps25) (Dermal papilla-derived protein 9) (ELL-associated protein of 20 kDa) (ESCRT-II complex subunit VPS25), VPS25, DERP9 EAP20

Background: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q9BRG1

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Recombinant Human Vacuolar protein-sorting-associated protein 25 protein (1-176AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Signal transduction

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