TRIM28 Antibody | CSB-PA05254A0Rb

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SKU:
CSB-PA05254A0Rb
Availability:
3 to 7 Working Days
  • TRIM28 Antibody
  • Western Blot<br />
 Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate<br />
 All lanes: TRIM28 antibody at 1.5µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 89, 80 kDa<br />
 Observed band size: 89 kDa<br />
  • Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA05254A0Rb at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA05254A0Rb at dilution of 1:100
  • Immunoprecipitating TRIM28 in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG (1µg) instead of CSB-PA05254A0Rb in Hela whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA05254A0Rb (6µg) + Hela whole cell lysate (500µg) <br />
 Lane 3: Hela whole cell lysate (10µg) <br />
€315.80 - €488.70

Description

TRIM28 Antibody | CSB-PA05254A0Rb | Cusabio

TRIM28 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: TRIM28

Aliases: Transcription intermediary factor 1-beta (TIF1-beta) (E3 SUMO-protein ligase TRIM28) (EC 2.3.2.27) (KRAB-associated protein 1) (KAP-1) (KRAB-interacting protein 1) (KRIP-1) (Nuclear corepressor KAP-1) (RING finger protein 96) (RING-type E3 ubiquitin transferase TIF1-beta) (Tripartite motif-containing protein 28), TRIM28, KAP1 RNF96 TIF1B

Background: Forms a complex with a KRAB-domain transcription factor and increases the efficiency of KRAB-mediated repression. Silences transcription through an interaction with HP1 proteins. Acts as a corepressor of transcription for the KRAB zinc finger proteins and as a moderator of the repression activity. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of the Alpha-1-acid glycoprotein gene.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q13263

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Transcription intermediary factor 1-beta protein (22-291AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IP

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology

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