TJP2 Antibody | CSB-PA880071NA01HU

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CSB-PA880071NA01HU
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  • TJP2 Antibody
  • IHC image of CSB-PA880071NA01HU diluted at 1:600 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of U251 cells with CSB-PA880071NA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate<br />
 All lanes: TJP2 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 134, 118, 112, 132, 116, 131, 138 kDa<br />
 Observed band size: 150 kDa<br />
€315.80 - €488.70

Description

TJP2 Antibody | CSB-PA880071NA01HU | Cusabio

TJP2 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: TJP2

Aliases: Tight junction protein ZO-2 (Tight junction protein 2) (Zona occludens protein 2) (Zonula occludens protein 2), TJP2, X104 ZO2

Background: Plays a role in tight junctions and adherens junctions.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q9UDY2

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence from Human Tight junction protein ZO-2 protein (1165-1183AA)

Immunogen Species: Homo sapiens (Human)

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:200-1:500

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:500-1:1000

Dilution Ratio4: IF:1:200-1:500

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Signal transduction

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