SOX2 Antibody | CSB-PA16539A0Rb

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SKU:
CSB-PA16539A0Rb
Availability:
3 to 7 Working Days
  • SOX2 Antibody
  • Western blot<br />
 All lanes: Transcription factor SOX-2 antibody at 2µg/ml + Mouse kidney tissue<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/15000 dilution<br />
 Predicted band size: 34 kDa<br />
 Observed band size: 34 kDa<br />
  • Western Blot<br />
 All lanes: Transcription factor SOX-2 antibody at 6µg/ml + 293T whole cell lysate<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/15000 dilution<br />
 Predicted band size: 34 kDa<br />
 Observed band size: 34 kDa<br />
  • IHC image of CSB-PA16539A0Rb diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Western Blot<br />
 Positive WB detected in: Mouse brain tissue<br />
 All lanes: SOX2 antibody at 3.5µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 34 kDa<br />
 Observed band size: 34 kDa<br />
  • Western Blot<br />
 Positive WB detected in: Zebrafish 40μg, 20μg, 10µg<br />
 All lanes: Sox2 antibody at 3µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 35 kDa<br />
 Observed band size: 35 kDa<br />
  • IHC image of CSB-PA16539A0Rb diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of HepG2 cells with CSB-PA16539A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

SOX2 Antibody | CSB-PA16539A0Rb | Cusabio

SOX2 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: SOX2

Aliases: Transcription factor SOX-2, SOX2

Background: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 By similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal By similarity. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P48431

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Recombinant Human Transcription factor SOX-2 protein (1-317AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Epigenetics & Nuclear Signaling;Neuroscience;Developmental biology;Signal transduction;Stem cells

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