Recombinant Xenopus laevis GATA-binding factor 1-A (gata1-a) | CSB-EP326431XBE

Recombinant Xenopus laevis GATA-binding factor 1-A (gata1-a) | CSB-EP326431XBE | Cusabio

Alternative Name(s): Transcription factor xGATA-1A

Gene Names: gata1-a

Research Areas: Epigenetics and Nuclear Signaling

Organism: Xenopus laevis (African clawed frog)

AA Sequence: MDYTTLTTQDPDPNYTESGLASTSEDSQFLYGLGGESSPGHYGGAVSSRAVGGFRHSPVFQTFPLHWPETSAGIPSNLTAYGRSTGTLSFYPSAASALGPITSPPLYSASSFLLGSAPPAEREGSPKFLETLKTERASPLTSDLLPLEPRSPSILQVGYIGGGGQEFSLFQSTEDRECVNCGATVTPLWRRDMSGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCSNCHTSTTTLWRRNASGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKVSSRSKKKKQLDNPFEPPKAGVEEPSPYPFGPLLFHGQMPPMGHMINPPHHFLQSPRISHSAPAVSYRQAASGVTPP

Source: E.coli

Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Expression Region: 1-359aa

Sequence Info: Full Length

MW: 59 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.

Reference: "Expression of GATA-binding proteins during embryonic development in Xenopus laevis." Zon L.I., Mather C., Burgess S., Bolce M.E., Harland R.M., Orkin S.H. Proc. Natl. Acad. Sci. U.S.A. 88:10642-10646(1991)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Transcription factor that acts synergistically with tal1/scl and lmo2 to specify embryonic dorsal mesoderm to a hematopoietic fate.

Involvement in disease:

Subcellular Location: Nucleus

Protein Families:

Tissue Specificity: Expressed in the developing ventral blood island, and in both tadpole and adult erythrocytes.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P23767

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A