Recombinant Xanthomonas campestris pv. campestris NAD-dependent protein deacetylase (cobB) | CSB-EP536037XAZ

Recombinant Xanthomonas campestris pv. campestris NAD-dependent protein deacetylase (cobB) | CSB-EP536037XAZ | Cusabio

Alternative Name(s): Regulatory protein SIR2 homolog

Gene Names: cobB

Research Areas: Others

Organism: Xanthomonas campestris pv. campestris (strain B100)

AA Sequence: MTVAITQTGPALQEFIKRHQRLFVLSGAGCSTDSGIPDYRDLHGGWKRPQPVTFQAFMGELSTRQRYWARSLVGWPRFGLARPNATHHALAALEARGQLELLLTQNVDRLHQAAGSQAVIDLHGRLDVVRCMGCEQRMPRTEFQLLLERDNPGWADLEAAQAPDGDADLDSVAFDNFVVPACPACGCVLKPDVVFFGENVPRERVERAFAHLQAADAVLVVGSSLMVYSGFRFVQAAARAGLPIAALNFGRTRADDLLSLKVEQSCAQALAFLQQPPDPLHTATARYHSARSA

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-293aa

Sequence Info: Full Length

MW: 48.1 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.

Reference: "The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.J. Biotechnol. 134:33-45(2008)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form.

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Sirtuin family, Class II subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: B0RM75

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A