Recombinant Vibrio cholerae serotype O1 Zona occludens toxin (zot) | CSB-EP334425VEX

Recombinant Vibrio cholerae serotype O1 Zona occludens toxin (zot) | CSB-EP334425VEX | Cusabio

Alternative Name(s): Zonular occludens toxin Short name: Zot

Gene Names: zot

Research Areas: Microbiology

Organism: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

AA Sequence: MSIFIHHGAPGSYKTSGALWLRLLPAIKSGRHIITNVRGLNLERMAKYLKMDVSDISIEFIDTDHPDGRLTMARFWHWARKDAFLFIDECGRIWPPRLTVTNLKALDTPPDLVAEDRPESFEVAFDMHRHHGWDICLTTPNIAKVHNMIREAAEIGYRHFNRATVGLGAKFTLTTHDAANSGQMDSHALTRQVKKIPSPIFKMYASTTTGKARDTMAGTALWKDRKILFLFGMVFLMFSYSFYGLHDNPIFTGGNDATIESEQSEPQSKATVGNAVGSKAVAPASFGFCIGRLCVQDGFVTVGDERYRLVDNLDIPYRGLWATGHHIYKDTLTVFFETESGSVPTELFASSYRYKVLPLPDFNHFVVFDTFAAQALWVEVKRGLPIKTENDKKGLNSIF

Source: E.coli

Tag Info: N-terminal 10xHis-tagged

Expression Region: 1-399aa

Sequence Info: Full Length

MW: 48.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Increases the permeability of the small intestine mucosa by affecting the structure of intercellular tight junctions (zonula occludens)

Reference: "Cloning of a gene (zot) encoding a new toxin produced by Vibrio cholerae."Baudry B., Fasano A., Ketley J., Kaper J.B.Infect. Immun. 60:428-434(1992)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Increases the permeability of the small intestine mucosa by affecting the structure of intercellular tight junctions (zonula occludens).

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P38442

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A