Recombinant Trypanosoma cruzi Trypanothione reductase (TPR) | CSB-YP333370TQY

Recombinant Trypanosoma cruzi Trypanothione reductase (TPR) | CSB-YP333370TQY | Cusabio

Alternative Name(s): N(1),N(8)-bis(glutathionyl)spermidine reductase

Gene Names: TPR

Research Areas: Others

Organism: Trypanosoma cruzi

AA Sequence: MMSKIFDLVVIGAGSGGLEAAWNAATLYKKRVAVIDVQMVHGPPFFSALGGTCVNVGCVPKKLMVTGAQYMEHLRESAGFGWEFDRTTLRAEWKKLIAVKDEAVLNINKSYEEMFRDTEGLEFFLGWGSLESKNVVNVRESADPASAVKERLETENILLASGSWPHMPNIPGIEHCISSNEAFYLPEPPRRVLTVGGGFISVEFAGIFNAYKPKDGQVTLCYRGEMILRGFDHTLREELTKQLTANGIQILTKENPAKVELNADGSKSVTFESGKKMDFDLVMMAIGRSPRTKDLQLQNAGVMIKNGGVQVDEYSRTNVSNIYAIGDVTNRVMLTPVAINEAAALVDTVFGTNPRKTDHTRVASAVFSIPPIGTCGLIEEVASKRYEVVAVYLSSFTPLMHNISGSKYKTFVAKIITNHSDGTVLGVHLLGDNAPEIIQGVGICLKLNAKISDFYNTIGVHPTSAEELCSMRTPSYYYVKGEKMEKPSEASL

Source: Yeast

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-492aa

Sequence Info: Full Length

MW: 55.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Trypanothione is the parasite analog of glutathione; this enzyme is the equivalent of glutathione reductase.

Reference: "Cloning, sequencing, overproduction and purification of trypanothione reductase from Trypanosoma cruzi."Sullivan F.X., Walsh C.T.Mol. Biochem. Parasitol. 44:145-148(1991)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Trypanothione is the parasite analog of glutathione; this enzyme is the equivalent of glutathione reductase.

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Class-I pyridine nucleotide-disulfide oxidoreductase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P28593

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A