Recombinant Staphylococcus aureus Phenol-soluble modulin alpha 1 peptide (psmA1) | CSB-YP430155SUL

Recombinant Staphylococcus aureus Phenol-soluble modulin alpha 1 peptide (psmA1) | CSB-YP430155SUL | Cusabio

Alternative Name(s): psmA1; MW0406.4; Phenol-soluble modulin alpha 1 peptide

Gene Names: psmA1

Research Areas: Cell Biology

Organism: Staphylococcus aureus (strain MW2)

AA Sequence: MGIIAGIIKVIKSLIEQFTGK

Source: Yeast

Tag Info: N-terminal 6xHis-sumostar-tagged

Expression Region: 1-21aa

Sequence Info: Full Length

MW: 24.3 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Peptide which can recruit, activate and subsequently lyse human neutrophils, thus eliminating the main cellular defense against infection. Stimulates the secretion of the chemotactic factor interleukin-8. The ensuing activation process triggers an inflammatory response in the host, thus contributing greatly to virulence. Also possesses hemolytic activity, which may contribute to the development of disease.

Reference: "Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA." Wang R., Braughton K.R., Kretschmer D., Bach T.-H.L., Queck S.Y., Li M., Kennedy A.D., Dorward D.W., Klebanoff S.J., Peschel A., DeLeo F.R., Otto M. Nat. Med. 13:1510-1514(2007)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Peptide which can recruit, activate and subsequently lyse human neutrophils, thus eliminating the main cellular defense against infection. Stimulates the secretion of the chemotactic factor interleukin-8 (IL-8). The ensuing activation process triggers an inflammatory response in the host, thus contributing greatly to virulence. Also possesses hemolytic activity, which may contribute to the development of disease.

Involvement in disease:

Subcellular Location:

Protein Families: Phenol-soluble modulin alpha peptides family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: A9JX05

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A