Recombinant Saccharomyces cerevisiae Lanosterol 14-alpha demethylase (ERG11), partial | CSB-EP006459SVG1

Recombinant Saccharomyces cerevisiae Lanosterol 14-alpha demethylase (ERG11), partial | CSB-EP006459SVG1 | Cusabio

Alternative Name(s): CYPLI Cytochrome P450 51 Cytochrome P450-14DM Cytochrome P450-LIA1 Sterol 14-alpha demethylase

Gene Names: ERG11

Research Areas: Others

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

AA Sequence: MSATKSIVGEALEYVNIGLS

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-20aa

Sequence Info: Extracellular Domain

MW: 29.1 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Reference: "Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae."Kalb V.F., Woods C.W., Turi T.G., Dey C.R., Sutter T.R., Loper J.C.DNA 6:529-537(1987)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Involvement in disease:

Subcellular Location: Membrane, Single-pass membrane protein

Protein Families: Cytochrome P450 family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P10614

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A