Recombinant Rhesus Macaque Tumor necrosis factor protein (TNF), partial, (Active) | CSB-AP003221MOW

Recombinant Rhesus Macaque Tumor necrosis factor protein (TNF) ,partial, (Active) | CSB-AP003221MOW | Cusabio

Protein Description: Partial

Alternative Name (s) : Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, , NTF

Gene Names: TNF,TNFA,TNFSF2

Research Areas: Cancer

Species: Macaca mulatta (Rhesus macaque)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 77-233aa

Sequence Info: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of ＞ 2.0 × 107 IU/mg in the presence of actinomycin D.

MW: 17.3 kDa

Purity: >95% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {ECO:0000250}.

PubMed ID: 7561102; 15269276;

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.; FUNCTION

Involvement in disease:

Subcellular Location: Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLULAR LOCATION: C-domain 1: Secreted, SUBCELLULAR LOCATION: C-domain 2: Secreted

Protein Families: Tumor necrosis factor family

Tissue Specificity:

Paythway:

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 5 % trehalose

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P48094

Uniprot Entry Name: TNFA_MACMU

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A