Recombinant Rat Guanidinoacetate N-methyltransferase (Gamt) | CSB-EP009227RA

Recombinant Rat Guanidinoacetate N-methyltransferase (Gamt) | CSB-EP009227RA | Cusabio

Alternative Name(s): Gamt; Guanidinoacetate N-methyltransferase; EC 2.1.1.2

Gene Names: Gamt

Research Areas: Others

Organism: Rattus norvegicus (Rat)

AA Sequence: MSSSAASPLFAPGEDCGPAWRAAPAAYDTSDTHLQILGKPVMERWETPYMHSLAAAAASRGGRVLEVGFGMAIAASRVQQAPIKEHWIIECNDGVFQRLQNWALKQPHKVVPLKGLWEEEAPTLPDGHFDGILYDTYPLSEETWHTHQFNFIKTHAFRLLKPGGILTYCNLTSWGELMKSKYTDITAMFEETQVPALLEAGFQRENICTEVMALVPPADCRYYAFPQMITPLVTKH

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-236aa

Sequence Info: Full Length

MW: 30.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance:

Reference: crystal structures of guanidinoacetate methyltransferase ternary complexes.Komoto J., Yamada T., Takata Y., Konishi K., Ogawa H., Gomi T., Fujioka M., Takusagawa F.Biochemistry 43:14385-14394(2004)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.

Involvement in disease:

Subcellular Location:

Protein Families: Class I-like SAM-binding methyltransferase superfamily, RMT2 methyltransferase family

Tissue Specificity: Expressed in hepatic primordium in the embryo as soon as 12.5 days. In the adult, high levels of expression are found in liver and pancreas. Ubiquitously expressed in neuronal and glial cells in the brain.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P10868

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: N/A

STRING Database Link: STRING

OMIM Database Link: N/A