Recombinant Prevotella intermedia Alkaline phosphatase | CSB-EP001633EYGd1

Recombinant Prevotella intermedia Alkaline phosphatase | CSB-EP001633EYGd1 | Cusabio

Alternative Name(s): PiALP

Gene Names: N/A

Research Areas: Others

Organism: Prevotella intermedia

AA Sequence: TDMLAVSVSSTDAIGHKYGT

Source: E.coli

Tag Info: N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged

Expression Region: 1-20aa

Sequence Info: Full Length

MW: 32.1 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Acts against tyrosine-phosphatases.

Reference: "Purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium Prevotella intermedia." Ansai T., Awano S., Chen X., Fuchi T., Arimoto T., Akifusa S., Takehara T. FEBS Lett. 428:157-160(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Acts against tyrosine-phosphatases.

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P81800

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A