Recombinant Penicillium citrinum Nuclease P1 | CSB-EP328459PEUa0

Recombinant Penicillium citrinum Nuclease P1 | CSB-EP328459PEUa0 | Cusabio

Alternative Name(s): Deoxyribonuclease P1 (Endonuclease P1)

Gene Names: N/A

Research Areas: Others

Organism: Penicillium citrinum

AA Sequence: WGALGHATVAYVAQHYVSPEAASWAQGILGSSSSSYLASIASWADEYRLTSAGKWSASLHFIDAEDNPPTNCNVDYERDCGSSGCSISAIANYTQRVSDSSLSSENHAEALRFLVHFIGDMTQPLHDEAYAVGGNKINVTFDGYHDNLHSDWDTYMPQKLIGGHALSDAESWAKTLVQNIESGNYTAQAIGWIKGDNISEPITTATRWASDANALVCTVVMPHGAAALQTGDLYPTYYDSVIDTIELQIAKGGYRLANWINEIHGSEIAK

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-270aa

Sequence Info: Full Length

MW: 33.2 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Hydrolyzes only single-stranded DNA and RNA without apparent specificity for bases.

Reference: "Recognition of single-stranded DNA by nuclease P1: high resolution crystal structures of complexes with substrate analogs." Romier C., Dominguez R., Lahm A., Dahl O., Suck D. Proteins 32:414-424(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-81?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 5? for up to one week.

Function: Hydrolyzes only single-stranded DNA and RNA without apparent specificity for bases.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Nuclease type I family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P24289

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A