Recombinant Mouse C-C motif chemokine 12 (Ccl12) | CSB-RP091394m

Recombinant Mouse C-C motif chemokine 12 (Ccl12) | CSB-RP091394m | Cusabio

Alternative Name(s): MCP-1-related chemokine;Monocyte chemoattractant protein 5Monocyte chemotactic protein 5 ;MCP-5Small-inducible cytokine A12

Gene Names: Ccl12

Research Areas: Others

Organism: Mus musculus (Mouse)

AA Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 23-104aa

Sequence Info: Full Length of Mature Protein

MW: 13.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Chotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

Reference: Distinct expression and function of the novel mouse chemokine monocyte chemotactic protein-5 in lung allergic inflammation.Jia G.-Q., Gonzalo J.A., Lloyd C., Kremer L., Lu L., Martinez A.C., Wershil B.K., Gutierrez-Ramos J.-C.J. Exp. Med. 184:1939-1951(1996)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Intercrine beta (chemokine CC) family

Tissue Specificity: Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q62401

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A