Recombinant Measles virus Hemagglutinin glycoprotein (H), partial | CSB-EP357366MCQ1

Recombinant Measles virus Hemagglutinin glycoprotein (H), partial | CSB-EP357366MCQ1 | Cusabio

Alternative Name(s): H; Hemagglutinin glycoprotein

Gene Names: H

Research Areas: Others

Organism: Measles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus)

AA Sequence: EDSITIPYQGSGKGVSFQLVKLGVWKSPTDMQSWVPLSTDDPVIDRLYLSSHRGVIADNQAKWAVPTTRTDDKLRMETCFQQACKGKIQALCENPEWAPLKDNRIPSYGVLSVDLSLTVELKIKIASGFGPLITHGSGMDLYKSNHNNVYWLTIPPMKNLALGVINTLEWIPRFKVSPYLFNVPIKEAGEDCHAPTYLPAEVDGDVKLSSNLVILPGQDLQYVLATYDTSRVEHAVVYYVYSPSRSFSYFYPFRLPIKGVPIELQVECFTWDQKLWCRHFCVLADSESGGHITHSGMEGMGVSCTVTREDGTNRR

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 303-617aa

Sequence Info: Partial

MW: 39.3 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Attaches the virus to cell receptors and thereby initiating infection. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. May use human CD46 and/or SLAMF1 as receptors for viral entry into the cell. The high degree of interaction between H and MCP/CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis.

Reference: "Mutated and hypermutated genes of persistent measles viruses which caused lethal human brain diseases." Cattaneo R., Schmid A., Spielhofer P., Kaelin K., Baczko K., Meulen V., Pardowitz J., Flanagan S., Rima B.K., Udem S.A. Virology 173:415-425(1989)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Attaches the virus to cell receptors and thereby initiating infection. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. May use human CD46 and/or SLAMF1 as receptors for viral entry into the cell. The high degree of interaction between H and MCP/CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis.

Involvement in disease:

Subcellular Location: Virion membrane, Single-pass type II membrane protein, Host cell membrane, Single-pass type II membrane protein

Protein Families: Paramyxoviruses hemagglutinin-neuraminidase family, Non-sialidase subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P08362

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A