Recombinant Human xcitatory amino acid transporter 3 (SLC1A1), partial | CSB-EP021432HU

Recombinant Human xcitatory amino acid transporter 3 (SLC1A1), partial | CSB-EP021432HU | Cusabio

Alternative Name(s): Excitatory amino-acid carrier 1 Neuronal and epithelial glutamate transporter Sodium-dependent glutamate/aspartate transporter 3 Solute carrier family 1 member 1

Gene Names: SLC1A1

Research Areas: Neuroscience

Organism: Homo sapiens (Human)

AA Sequence: AEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 430-524aa

Sequence Info: Partial

MW: 37.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Transports L-glutamate, L- and D-aspartate and L-cystein (PubMed:21123949). Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5

Reference: "Loss-of-function mutations in the glutamate transporter SLC1A1 cause human dicarboxylic aminoaciduria."Bailey C.G., Ryan R.M., Thoeng A.D., Ng C., King K., Vanslambrouck J.M., Auray-Blais C., Vandenberg R.J., Broer S., Rasko J.E.J. Clin. Invest. 121:446-453(2011)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate

Involvement in disease: Dicarboxylic aminoaciduria (DCBXA); Schizophrenia 18 (SCZD18)

Subcellular Location: Cell membrane, Multi-pass membrane protein, Apical cell membrane, Multi-pass membrane protein

Protein Families: Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A1 subfamily

Tissue Specificity: Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).

Paythway: Glutamatergicsynapse

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P43005

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM