Recombinant Human Trefoil factor 1 protein (TFF1) (Active) | CSB-AP000341HU

Recombinant Human Trefoil factor 1 protein (TFF1) (Active) | CSB-AP000341HU | Cusabio

Protein Description: Full Length of Mature Protein

Alternative Name (s) : Breast cancer estrogen-inducible protein, Polypeptide P1.A, hP1.A, Protein pS2,

Gene Names: TFF1,BCEI,PS2

Research Areas: Signal Transduction

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 25-84aa

Sequence Info: EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of ＞ 100 IU/mg.

MW: 6.7 kDa

Purity: >97% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine. {ECO:0000269|PubMed:16308573}.

PubMed ID: 6324130; 3838275; 3822834; 2311759; 2185238; 10950923; 10830953; 15489334; 3146413; 15924415; 16308573; 3261981; 15340161; 3041593; 16718800; 17143957; 17242463; 8521850; 9096235; 10982763; 16697734

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Involvement in disease:

Subcellular Location: Secreted

Protein Families:

Tissue Specificity: Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level) . Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level) . Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level) . Detected in urine (at protein level) . Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level) .

Paythway: Estrogensignalingpathway

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P04155

Uniprot Entry Name: TFF1_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM