Recombinant Human Serotonin N-acetyltransferase (AANAT) | CSB-EP623090HU

Recombinant Human Serotonin N-acetyltransferase (AANAT) | CSB-EP623090HU | Cusabio

Alternative Name(s): Aralkylamine N-acetyltransferase

Gene Names: AANAT

Research Areas: Neuroscience

Organism: Homo sapiens (Human)

AA Sequence: MSTQSTHPLKPEAPRLPPGIPESPSCQRRHTLPASEFRCLTPEDAVSAFEIEREAFISVLGVCPLYLDEIRHFLTLCPELSLGWFEEGCLVAFIIGSLWDKERLMQESLTLHRSGGHIAHLHVLAVHRAFRQQGRGPILLWRYLHHLGSQPAVRRAALMCEDALVPFYERFSFHAVGPCAITVGSLTFMELHCSLRGHPFLRRNSGC

Source: E.coli

Tag Info: N-terminal 10xHis-GST-tagged

Expression Region: 1-207aa

Sequence Info: Full Length

MW: 51.8 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

Reference: "cAMP regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation." Coon S.L., Weller J.L., Korf H.-W., Namboodiri M.A., Rollag M., Klein D.C. J. Biol. Chem. 276:24097-24107(2001)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Acetyltransferase family, AANAT subfamily

Tissue Specificity: Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q16613

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM