Recombinant Human Putative ATP-dependent RNA helicase DHX33 (DHX33), partial | CSB-EP884479HU1

Recombinant Human Putative ATP-dependent RNA helicase DHX33 (DHX33), partial | CSB-EP884479HU1 | Cusabio

Alternative Name(s): DEAH box protein 33 (DDX33)

Gene Names: DHX33

Research Areas: Others

Organism: Homo sapiens (Human)

AA Sequence: MPEEAGFPPAKRFRPGSGPPSRAGSFPPGRQVVMLLTAGSGGRGGGGGRRQQPPLAQPSASPYPEAVELQRRSLPIFQARGQLLAQLRNLDNAVLIGETGSGKTTQIPQYLYEGGISRQGIIAVTQPRRVAAISLATRVSDEKRTELGKLVGYTVRFDDVTSEDTRIKFLTDGMLLREAISDSLLRKYSCVILDEAHERTIHTDVLFGVVKAAQKRRKELGKLPLKVIVMSATMDVDLFSQYFNGAPVLYLEGRQHPIQVFYTKQPQNDYLHAALVSVFQIHQEAPSSQDILVFLTGQEEIEAMSKTCRDIAKHLPDGCPAMLVLPLYASLPYAQQLRVFQGAPKGYRKVIISTNIAETSITITGIKYVVDTGMVKAKKYNPDSGLEVLAVQRVSKTQAWQRTGRAGREDSGICYRLYTEDEFEKFDKMTVPEIQRCNLASVMLQLLAMK

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-450aa

Sequence Info: Partial

MW: 53.7 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. Important element of nucleolar organization.

Reference: "Polymorphisms in innate immunity genes and risk of childhood leukemia." Han S., Lan Q., Park A.K., Lee K.M., Park S.K., Ahn H.S., Shin H.Y., Kang H.J., Koo H.H., Seo J.J., Choi J.E., Ahn Y.O., Chanock S.J., Kim H., Rothman N., Kang D. Hum. Immunol. 71:727-730(2010)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. Important element of nucleolar organization.

Involvement in disease:

Subcellular Location: Nucleus, nucleolus, Nucleus, nucleoplasm

Protein Families: DEAD box helicase family, DEAH subfamily

Tissue Specificity:

Paythway: NOD-likereceptorsignalingpathway

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9H6R0

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM