Recombinant Human Melanoma antigen recognized by T-cells 1 (MLANA) | CSB-YP621968HU

Recombinant Human Melanoma antigen recognized by T-cells 1 (MLANA) | CSB-YP621968HU | Cusabio

Alternative Name(s): Antigen LB39-AAAntigen SK29-AAProtein Melan-A

Gene Names: MLANA

Research Areas: Cancer

Organism: Homo sapiens (Human)

AA Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

Source: Yeast

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-118aa

Sequence Info: Full Length

MW: 15.2 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Reference: Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor.Kawakami Y., Eliyahu S., Delgado C.H., Robbins P.F., Rivoltini L., Topalian S.L., Miki T., Rosenberg S.A.Proc. Natl. Acad. Sci. U.S.A.91:3515-3519(1994)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Involvement in disease:

Subcellular Location: Endoplasmic reticulum membrane, Single-pass type III membrane protein, Golgi apparatus, Golgi apparatus, trans-Golgi network membrane, Melanosome

Protein Families:

Tissue Specificity: Expression is restricted to melanoma and melanocyte cell lines and retina.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q16655

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM