Recombinant Human Insulin-like growth factor II protein (PP1446), partial | CSB-RP083844h

Recombinant Human Insulin-like growth factor II protein (PP1446), partial | CSB-RP083844h | Cusabio

Alternative Name(s): Somatomedin-AT3M-11-derived growth factor

Gene Names: PP1446

Research Areas: Metabolism

Organism: Homo sapiens (Human)

AA Sequence: ETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPAKSERDVSTPPTVLPDNFPRYPVGKFFQYDTWKQSTQRLRRGLPALLRARRGHVLAKELEAFREAKRHRPLIALPTQDPAHGGAPPEMASNRK

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 30-180aa

Sequence Info: Partial

MW: 44.1 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

Reference: Insulin-like growth factor II precursor gene organization in relation to insulin gene family.Dull T.J., Gray A., Hayflick J.S., Ullrich A.Nature 310:777-781(1984)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable).

Involvement in disease: Silver-Russell syndrome (SRS); Growth restriction, severe, with distinctive facies (GRDF)

Subcellular Location: Secreted

Protein Families: Insulin family

Tissue Specificity: Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye and pancreas.

Paythway: MAPKsignalingpathway

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P01344

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM