Recombinant Human Guanylate cyclase soluble subunit alpha-2 (GUCY1A2), partial | CSB-EP010049HU1

Recombinant Human Guanylate cyclase soluble subunit alpha-2 (GUCY1A2), partial | CSB-EP010049HU1 | Cusabio

Alternative Name(s): GCS-alpha-2

Gene Names: GUCY1A2

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: GDVAQQLWQGQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFLDIYKVETIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQMRIGIHSGSVLAGVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREESFTFIPRSREELPDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 500-732aa

Sequence Info: Partial

MW: 27.8 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Has guanylyl cyclase on binding to the beta-1 subunit. Isoform 2 acts as a negative regulator of guanylyl cyclase activity as it forms non-functional heterodimers with the beta subunits.

Reference: "Interaction proteome of human Hippo signaling: modular control of the co-activator YAP1." Hauri S., Wepf A., van Drogen A., Varjosalo M., Tapon N., Aebersold R., Gstaiger M. Mol Syst Biol 9:713-713(2013)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P33402

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A