Recombinant Human Endophilin-A2 (SH3GL1) | CSB-EP859949HUb0

Recombinant Human Endophilin-A2 (SH3GL1) | CSB-EP859949HUb0 | Cusabio

Alternative Name(s): EEN fusion partner of MLL (Endophilin-2) (Extra eleven-nineteen leukemia fusion gene protein) (EEN) (SH3 domain protein 2B) (SH3 domain-containing GRB2-like protein 1 CNSA1) (SH3D2B)

Gene Names: SH3GL1

Research Areas: Cell Biology

Organism: Homo sapiens (Human)

AA Sequence: MSVAGLKKQFYKASQLVSEKVGGAEGTKLDDDFKEMEKKVDVTSKAVTEVLARTIEYLQPNPASRAKLTMLNTVSKIRGQVKNPGYPQSEGLLGECMIRHGKELGGESNFGDALLDAGESMKRLAEVKDSLDIEVKQNFIDPLQNLCEKDLKEIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFEESKEVAETSMHNLLETDIEQVSQLSALVDAQLDYHRQAVQILDELAEKLKRRMREASSRPKREYKPKPREPFDLGEPEQSNGGFPCTTAPKIAASSSFRSSDKPIRTPSRSMPPLDQPSCKALYDFEPENDGELGFHEGDVITLTNQIDENWYEGMLDGQSGFFPLSYVEVLVPLPQ

Source: E.coli

Tag Info: N-terminal 10xHis-tagged

Expression Region: 1-368aa

Sequence Info: Full Length

MW: 45.0 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Implicated in endocytosis. May recruit other proteins to membranes with high curvature

Reference: "EEN encodes for a member of a new family of proteins containing an Src homology 3 domain and is the third gene located on chromosome 19p13 that fuses to MLL in human leukemia." So C.W., Caldas C., Liu M.-M., Chen S.-J., Huang Q.-H., Gu L.-J., Sham M.H., Wiedemann L.M., Chan L.C. Proc. Natl. Acad. Sci. U.S.A. 94:2563-2568(1997)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).

Involvement in disease: In some cases of acute leukemia, a translocation results in the formation of a KMT2A/MLL1-EEN fusion gene.

Subcellular Location: Cytoplasm, Early endosome membrane, Peripheral membrane protein, Cell projection, podosome

Protein Families: Endophilin family

Tissue Specificity: Ubiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.

Paythway: Endocytosis

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q99961

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM