Recombinant Human Cytoplasmic dynein 2 light intermediate chain 1 (DYNC2LI1) | CSB-EP844722HUe0

Recombinant Human Cytoplasmic dynein 2 light intermediate chain 1 (DYNC2LI1) | CSB-EP844722HUe0 | Cusabio

Alternative Name(s): CGI-60; Cytoplasmic dynein 2 light intermediate chain 1; D2LIC; DC2L1_HUMAN; DYNC2LI1; Dynein 2 light intermediate chain; Dynein, cytoplasmic 2, light intermediate chain 1; LIC3

Gene Names: DYNC2LI1

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: MPSETLWEIAKAEVEKRGINGSEGDGAEIAEKFVFFIGSKNGGKTTIILRCLDRDEPPKPTLALEYTYGRRAKGHNTPKDIAHFWELGGGTSLLDLISIPITGDTLRTFSLVLVLDLSKPNDLWPTMENLLQATKSHVDKVIMKLGKTNAKAVSEMRQKIWNNMPKDHPQDHELIDPFPVPLVIIGSKYDVFQDFESEKRKVICKTLRFVAHYYGASLMFTSKSEALLLKIRGVINQLAFGIDKSKSICVDQNKPLFITAGLDSFGQIGSPPVPENDIGKLHAHSPMELWKKVYEKLFPPKSINTLKDIKDPARDPQYAENEVDEMRIQKDLELEQYKRSSSKSWKQIELDS

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-352aa

Sequence Info: Full Length of Isoform 2

MW: 66.8 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis

Reference: "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Required for correct intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. Involved in the regulation of ciliary length.

Involvement in disease: Short-rib thoracic dysplasia 15 with polydactyly (SRTD15)

Subcellular Location: Cytoplasm, Cell projection, cilium, Cytoplasm, cytoskeleton, cilium basal body, Cytoplasm, cytoskeleton, cilium axoneme, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Protein Families: Dynein light intermediate chain family

Tissue Specificity: Expressed in bone, brain, kidney, and cartilage (PubMed:26077881, PubMed:26130459). Lower expression in heart, liver, lung, placenta and thymus (PubMed:26077881).

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q8TCX1

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM