Recombinant Human Cytoplasmic dynein 2 light intermediate chain 1 (DYNC2LI1) | CSB-EP844722HU

Recombinant Human Cytoplasmic dynein 2 light intermediate chain 1 (DYNC2LI1) | CSB-EP844722HU | Cusabio

Alternative Name(s): CGI-60; Cytoplasmic dynein 2 light intermediate chain 1; D2LIC; DC2L1_HUMAN; DYNC2LI1; Dynein 2 light intermediate chain; Dynein, cytoplasmic 2, light intermediate chain 1; LIC3

Gene Names: DYNC2LI1

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: MPSETLWEIAKAEVEKRGINGSEGDGAEIAEKFVFFIGSKNGGKTTIILRCLDRDEPPKPTLALEYTYGRRAKGHNTPKDIAHFWELGGGTSLLDLISIPITGDTLRTFSLVLVLDLSKPNDLWPTMENLLQATKSHVDKVIMKLGKTNAKAVSEMRQKIWNNMPKDHPQDHELIDPFPVPLVIIGSKYDVFQDFESEKRKVICKTLRFVAHYYGASLMFTSKSEALLLKIRGVINQLAFGIDKSKSICVDQNKPLFITAGLDSFGQIGSPPVPENDIGKLHAHSPMELWKKVYEKLFPPKSINTLKDIKDPARDPQYAENEVDEMRIQKDLELEQYKRSSSKSWKQIELDS

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 1-352aa

Sequence Info: Full Length of Isoform 2

MW: 44.8 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis (By similarity).

Reference: "Identification of a novel light intermediate chain (D2LIC) for mammalian cytoplasmic dynein 2."Grissom P.M., Vaisberg E.A., McIntosh J.R.Mol. Biol. Cell 13:817-829(2002)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Required for correct intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. Involved in the regulation of ciliary length.

Involvement in disease: Short-rib thoracic dysplasia 15 with polydactyly (SRTD15)

Subcellular Location: Cytoplasm, Cell projection, cilium, Cytoplasm, cytoskeleton, cilium basal body, Cytoplasm, cytoskeleton, cilium axoneme, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Protein Families: Dynein light intermediate chain family

Tissue Specificity: Expressed in bone, brain, kidney, and cartilage (PubMed:26077881, PubMed:26130459). Lower expression in heart, liver, lung, placenta and thymus (PubMed:26077881).

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q8TCX1

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM