Cusabio Active Proteins
Recombinant Human C-C motif chemokine 25 protein (CCL25) (Active) | CSB-AP000981HU
- SKU:
- CSB-AP000981HU
- Availability:
- 5 to 10 Working Days
Description
Recombinant Human C-C motif chemokine 25 protein (CCL25) (Active) | CSB-AP000981HU | Cusabio
Protein Description: Full Length of Mature Protein
Alternative Name (s) : Chemokine TECK, Thymus-expressed chemokine
Gene Names: CCL25,SCYA25,TECK
Research Areas: Immunology
Species: Homo sapiens (Human)
Source: E.Coli
Tag Info: Tag-Free
Expression Region: 24-150aa
Sequence Info: M+QGVFEDCCL AYHYPIGWAV LRRAWTYRIQ EVSGSCNLPA AIFYLPKRHR KVCGNPKSRE VQRAMKLLDA RNKVFAKLHH NTQTFQAGPH AVKKLSSGNS KLSSSKFSNP ISSSKRNVSL LISANSGL
Biological Activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
MW: 14.3 kDa
Purity: >97% as determined by SDS-PAGE and HPLC.
Endotoxin: Less than 1.0 EU/µg as determined by LAL method.
Relevance: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
PubMed ID: 9285413; 14702039; 15057824; 15489334; 10229797; 23341447
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Function: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Involvement in disease:
Subcellular Location: Secreted
Protein Families: Intercrine beta (chemokine CC) family
Tissue Specificity: Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Paythway: Chemokinesignalingpathway
Form: Lyophilized powder
Buffer: Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: O15444
Uniprot Entry Name: CCL25_HUMAN
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM