Recombinant Human Beta-defensin 103 protein (DEFB103A) (Active) | CSB-AP003051HU

Recombinant Human Beta-defensin 103 protein (DEFB103A) (Active) | CSB-AP003051HU | Cusabio

Protein Description: Full Length of Mature Protein

Alternative Name (s) : Beta-defensin 3, DEFB-3, HBD3, hBD-3, Defensin, beta 103

Gene Names: DEFB103A,BD3,DEFB103,DEFB3;,DEFB103B

Research Areas: Microbiology

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 23-67aa

Sequence Info: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 μg/ml, corresponding to a specific activity of ＞ 33.3 IU/mg.

MW: 5.2 kDa

Purity: >98% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. {ECO:0000269|PubMed:11085990}.

PubMed ID: 11085990; 11702237; 11223260; 11741980

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Beta-defensin family

Tissue Specificity: Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.

Paythway:

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130 mM NaCl

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P81534

Uniprot Entry Name: D103A_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM