Recombinant Human Alanine aminotransferase 1 (GPT) | CSB-EP009864HU

Recombinant Human Alanine aminotransferase 1 (GPT) | CSB-EP009864HU | Cusabio

Alternative Name(s): Glutamate pyruvate transaminase 1 ;GPT 1;Glutamic--alanine transaminase 1;Glutamic--pyruvic transaminase 1

Gene Names: GPT

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: ASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAKFTLEYS

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 2-496aa

Sequence Info: Full Length of Mature Protein

MW: 58.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles .

Reference: "Joubert Syndrome in French Canadians and Identification of Mutations in CEP104." Care4Rare Canada Consortium Srour M., Hamdan F.F., McKnight D., Davis E., Mandel H., Schwartzentruber J., Martin B., Patry L., Nassif C., Dionne-Laporte A., Ospina L.H., Lemyre E., Massicotte C., Laframboise R., Maranda B., Labuda D., Decarie J.C., Rypens F. Michaud J.L. Am. J. Hum. Genet. 97:744-753(2015)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily

Tissue Specificity: Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P24298

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM