Recombinant Geobacillus stearothermophilus Glycerol-1-phosphate dehydrogenase [NAD (P) +] (egsA) | CSB-EP455307GFM

Recombinant Geobacillus stearothermophilus Glycerol-1-phosphate dehydrogenase [NAD (P) +] (egsA) | CSB-EP455307GFM | Cusabio

Alternative Name(s): Enantiomeric glycerophosphate synthase sn-glycerol-1-phosphate dehydrogenase

Gene Names: egsA

Research Areas: Others

Organism: Geobacillus stearothermophilus (Bacillus stearothermophilus)

AA Sequence: MNQTLEEVVSLARQCGCGHRHYDIPIEQMVVGREAFARLVAYLRHKRYERVAIVADDHTFAAVERSLCDQLENGSIRYTVCLVQPDENGDVIADERSIVQVLLETPDDVDVLIAVGAGTIHDITRFSSYKMRIPFISVPTAPSVDGFTSMGAPLIIRGVKKTIQAQAPIAVFAHTGVLCQSPKEMIAAGFGDMVAKYTSLADWQFAHWMADEPYCPFVHQLTEQSLQTCVDHIDDIAAGGEQGIRVLMDALLQSGIAMLLMGQSYSASGAEHHLSHYWEMEFLRQKKRQVLHGAKVGVSTPIIIEHYQRVFWPLLNELEKRPKSMDEATWERLKANTASIRELLESLPSPERIRTMLAKVGGAIAPEQLGIDPQLVERSLREAHRLRLNRFTMLYFLNELIFVE

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-404aa

Sequence Info: Full Length

MW: 61.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species.

Reference: "Hemicellulose utilization cluster in Geobacillus stearothermophilus strain T-6."Shoham Y., Shulami S., Ben-David A., Langut Y.Submitted (JUN-2008)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species.

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Glycerol-1-phosphate dehydrogenase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: B3EYN8

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A