Recombinant Escherichia coli Murein hydrolase activator NlpD (nlpD) | CSB-EP359913ENV

Recombinant Escherichia coli Murein hydrolase activator NlpD (nlpD) | CSB-EP359913ENV | Cusabio

Alternative Name(s): nlpD; b2742; JW2712Murein hydrolase activator NlpD

Gene Names: nlpD

Research Areas: Microbiology

Organism: Escherichia coli (strain K12)

AA Sequence: CSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 26-379aa

Sequence Info: Full Length of Mature Protein

MW: 53.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.

Reference: "LytM-domain factors are required for daughter cell separation and rapid ampicillin-induced lysis in Escherichia coli."Uehara T., Dinh T., Bernhardt T.G.J. Bacteriol. 191:5094-5107(2009).

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.

Involvement in disease:

Subcellular Location: Cell inner membrane, Lipid-anchor

Protein Families: E.coli NlpD/Haemophilus LppB family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P0ADA3

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A