Cusabio Escherichia coli Recombinants
Recombinant Escherichia coli GTP cyclohydrolase-2 (ribA) | CSB-RP167474Ba
- SKU:
- CSB-RP167474Ba
- Availability:
- 13 - 23 Working Days
Description
Recombinant Escherichia coli GTP cyclohydrolase-2 (ribA) | CSB-RP167474Ba | Cusabio
Alternative Name(s): GTP cyclohydrolase II
Gene Names: ribA
Research Areas: Others
Organism: Escherichia coli (strain K12)
AA Sequence: MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHTPVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKIRAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTEAGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK
Source: E.coli
Tag Info: N-terminal 6xHis-tagged
Expression Region: 1-196aa
Sequence Info: Full Length
MW: 25.8 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.
Reference: Biosynthesis of riboflavin studies on the mechanism of GTP cyclohydrolase II.Ritz H., Schramek N., Bracher A., Herz S., Eisenreich W., Richter G., Bacher A.J. Biol. Chem. 276:22273-22277(2001)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.
Involvement in disease:
Subcellular Location:
Protein Families: GTP cyclohydrolase II family
Tissue Specificity:
Paythway:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P0A7I7
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A