Recombinant Drosophila melanogaster Protein bottleneck (bnk) | CSB-EP328169DLU

Recombinant Drosophila melanogaster Protein bottleneck (bnk) | CSB-EP328169DLU | Cusabio

Alternative Name(s): bnk; CG1480; Protein bottleneck

Gene Names: bnk

Research Areas: Others

Organism: Drosophila melanogaster (Fruit fly)

AA Sequence: MSISTFNFQFYNYKLSPSSPGFGSTASRSSSSCISELEMDIDEDMSAQPTITSTPKPRFTSQLAVELAKTEPGNGISPLRPKLHTAQKRWSMELREKVLEMSKRNNGQEKPQTARQQEQRQPQEQPLQQEELQHQQQEPTVTDKINFFNKLTNTFESGFSKLMPQASSTNNRFIAMLRTARPQNVATTTANSSTANSFLGSDHSLSGSVTGQVPPPKPKRLSATTAQFATPHVPAMGVGKGGSQRKCSLRRKPSMDKSRATISRQSSSASVRTQNHAIMEDLSLVVPVRLRIAEYEQRISMSA

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-303aa

Sequence Info: Full Length

MW: 37.6 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Acts as a regulator of the microfilament network governing cellularization of the embryo. Determines the timing of a key conformational transition in the cortical microfilament network: the proper coordination of membrane invagination and basal closure of the cells. To do this, bnk possibly physically links neighboring contractile units of the early cycle 14 microfilament network in a manner that prevents basal constriction until the proper stage has been reached. Bnk together with nullo and Sry-alpha may provide auxiliary functions, by acting both to stabilize a large and dynamic microfilament structure and regulate its functions.

Reference: "Bottleneck acts as a regulator of the microfilament network governing cellularization of the Drosophila embryo."Schejter E.D., Wieschaus E.Cell 75:373-385(1993)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Acts as a regulator of the microfilament network governing cellularization of the embryo. Determines the timing of a key conformational transition in the cortical microfilament network

Involvement in disease:

Subcellular Location: Cytoplasm, cytoskeleton

Protein Families:

Tissue Specificity: Restricted to the blastoderm.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P40794

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A