Recombinant Dog Arginine esterase | CSB-EP362734DO

Recombinant Dog Arginine esterase | CSB-EP362734DO | Cusabio

Alternative Name(s): Arginine esterase; EC 3.4.21.35

Gene Names: N/A

Research Areas: Others

Organism: Canis lupus familiaris (Dog) (Canis familiaris)

AA Sequence: IIGGRECLKNSQPWQVAVYHNGEFACGGVLVNPEWVLTAAHCANSNCEVWLGRHNLSESEDEGQLVQVRKSFIHPLYKTKVPRAVIRPGEDRSHDLMLLHLEEPAKITKAVRVMDLPKKEPPLGSTCYVSGWGSTDPETIFHPGSLQCVDLKLLSNNQCAKVYTQKVTKFMLCAGVLEGKKDTCKGDSGGPLICDGELVGITSWGATPCGKPQMPSLYTRVMPHLMWIKDTMKANT

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 25-260aa

Sequence Info: Full Length of Mature Protein

MW: 42 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: This serine protease is found in dog seminal plasma, its exact physiological function is not known. Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Reference: "Nucleotide sequence of the androgen-dependent arginine esterase mRNA of canine prostate."Chapdelaine P., Ho-Kim M.-A., Tremblay R.R., Dube J.Y.FEBS Lett. 232:187-192(1988)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: This serine protease is found in dog seminal plasma, its exact physiological function is not known.

Involvement in disease:

Subcellular Location:

Protein Families: Peptidase S1 family, Kallikrein subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P09582

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A