Recombinant Dirofilaria immitis Pepsin inhibitor Dit33 (DIT33) | CSB-EP638886DKS

Recombinant Dirofilaria immitis Pepsin inhibitor Dit33 (DIT33) | CSB-EP638886DKS | Cusabio

Alternative Name(s): /

Gene Names: DIT33

Research Areas: Developmental Biology

Organism: Dirofilaria immitis (Canine heartworm)

AA Sequence: SVINRHNKRFAGFSVAGIGGTAGCVVVDNKLFANSFYLRDLTTEEQRELAQYVEDSNQYKEEVKTSLEERRKGWQLARHGEKDAKVLSSLAEKKFPKPPKKPSFCSAGDTTQYYFDGCMVQNNKIYVGRMYVRDLTSDEINQLKTFDAKMTAYQKYLSSSIQQQVDSLFGDKSNLFNLFTDTRHETSSQPSDATTISTTTQAPVEPPETPHFCIAIY

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 18-234aa

Sequence Info: Full Length of Mature Protein

MW: 32.0 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Aspartyl protease inhibitor.

Reference: "Cloning and expression of DiT33 from Dirofilaria immitis: a specific and early marker of heartworm infection." Hong X.Q., Mejia J.S., Kumar S., Perler F.B., Carlow C.K.S. Parasitology 112:331-338(1996)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q27384

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A