Recombinant Dermatophagoides pteronyssinus Tropomyosin | CSB-EP517721DJH

Recombinant Dermatophagoides pteronyssinus Tropomyosin | CSB-EP517721DJH | Cusabio

Alternative Name(s): Allergen: Der p 10

Gene Names: N/A

Research Areas: Signal Transduction

Organism: Dermatophagoides pteronyssinus (European house dust mite)

AA Sequence: MEAIKNKMQAMKLEKDNAIDRAEIAEQKARDANLRAEKSEEEVRALQKKIQQIENELDQVQEQLSAANTKLEEKEKALQTAEGDVAALNRRIQLIEEDLERSEERLKIATAKLEEASQSADESERMRKMLEHRSITDEERMEGLENQLKEARMMAEDADRKYDEVARKLAMVEADLERAEERAETGESKIVELEEELRVVGNNLKSLEVSEEKAQQREEAHEQQIRIMTTKLKEAEARAEFAERSVQKLQKEVGRLEDELVHEKEKYKSISDELDQTFAELTGY

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-284aa

Sequence Info: Full Length

MW: 48.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Tropomyosin, in association with the troponin complex, plays a central role in the calcium dependent regulation of muscle contraction.

Reference: "Sequencing and high level expression in Escherichia coli of the tropomyosin allergen (Der p 10) from Dermatophagoides pteronyssinus." Asturias J.A., Arilla M.C., Gomez-Bayon N., Martinez J., Martinez A., Palacios R.Biochim. Biophys. Acta 1397:27-30(1998)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Tropomyosin, in association with the troponin complex, plays a central role in the calcium dependent regulation of muscle contraction.

Involvement in disease:

Subcellular Location:

Protein Families: Tropomyosin family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: O18416

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A