Recombinant Cyanophora paradoxa Chaperone protein dnaK (dnaK-A), partial | CSB-EP656399DZX

Recombinant Cyanophora paradoxa Chaperone protein dnaK (dnaK-A), partial | CSB-EP656399DZX | Cusabio

Alternative Name(s): HSP70 Heat shock 70 kDa protein Heat shock protein 70

Gene Names: dnaK-A

Research Areas: Signal Transduction

Organism: Cyanophora paradoxa

AA Sequence: GEVKDIVLLDVTPLSLGVETLGGVMSKIIVRNTTIPTQKSELFSTATDGQTNVEIHVLQGERGFAKDNKSLGTFRLDGIAPAPRGVPQIEVTFDIDANGILSVTAKDKGTGKEKSITITGASTLSQDEVERMVQEAEKNAAQDKENRQKIELRNQAE

Source: E.coli

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 377-533aa

Sequence Info: Partial

MW: 21.9 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Acts as a chaperone.

Reference: "The complete sequence of the cyanelle genome of Cyanophora paradoxa: the genetic complexity of a primitive plastid." Loeffelhardt W., Stirewalt V.L., Michalowski C.B., Annarella M., Farley J.Y., Schluchter W.M., Chung S., Newmann-Spallart C., Steiner J.M., Jakowitsch J., Bohnert H.J., Bryant D.A. (In) Schenk H.E.A., Herrmann R., Jeon K.W., Mueller N.E., Schwemmler W. (eds.); Eukaryotism and symbiosis, pp.40-48, Springer-Verlag, Heidelberg (1997)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Acts as a chaperone.

Involvement in disease:

Subcellular Location: Plastid, cyanelle

Protein Families: Heat shock protein 70 family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q37106

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A