Cusabio Virus & Bacteria Recombinants
Recombinant Bradyrhizobium sp. Formamidase (amiF) | CSB-EP397462BPF
- SKU:
- CSB-EP397462BPF
- Availability:
- 3 - 7 Working Days
Description
Recombinant Bradyrhizobium sp. Formamidase (amiF) | CSB-EP397462BPF | Cusabio
Alternative Name(s): Formamide amidohydrolase
Gene Names: amiF
Research Areas: Others
Organism: Bradyrhizobium sp. (strain ORS278)
AA Sequence: MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPEYSLHGLSMDTNPEIMCRLDGPEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSNGEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALIICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILAHGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMVAGTYRLPWEDQVKITDGTSCGFPAPTRVFGKMAKAAE
Source: E.coli
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 1-337aa
Sequence Info: Full Length
MW: 53.2 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide.
Reference: "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia."Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. Sadowsky M.Science 316:1307-1312(2007)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide.
Involvement in disease:
Subcellular Location:
Protein Families: Carbon-nitrogen hydrolase superfamily, Aliphatic amidase family
Tissue Specificity:
Paythway:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: A4Z3G9
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A