Recombinant Bacteroides vulgatus Alpha-1, 3-galactosidase B (glaB1) | CSB-EP406363BOP

Recombinant Bacteroides vulgatus Alpha-1, 3-galactosidase B (glaB1) | CSB-EP406363BOP | Cusabio

Alternative Name(s): Exo-alpha-galactosidase B1 (EC:3.2.1.22)

Gene Names: glaB1

Research Areas: Others

Organism: Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)

AA Sequence: CTTLSAQKVYEISAFGLKANSSKNASPVLQKALAKIKAEYKEGEKVILRFPEGRYEFHEKGAAVREYYISNHDQTNPKKVGIALEDMKNLTLDGQGSEFVFHGRMLPVSLLRSENCLLKNFSIDFENPHIAQVKIVENDPQDGIVFEPAPWVDYRIAKDSIFEAYGEGWTMRHSWGIAFDGDTKHLVYNTSDIGCPTKGASEVAPRRIHAPGWKDARLVPGTVVAMRGWGRPTPGIFLSHDVNTTIENVKVHYAEGMGLLAQLCENITLEKFGVCLKGDADPRYFTTQADATHFSGCKGKIVSCNGLYEGMMDDAINVHGTYLKVVKRVDDRTLVGRYMHGQSWGFEWGCPGDEVQFIRSNTMELVGKQNKIISIRPYDKEQTEGAREFLITFQEPVDQVINEQSGFGIENLTWTPEVLFSGNVIRNNRARGSLFSTPRKTIVENNLFDHTSGAAILLCGDCNGWFETGACRHVIIRKNRFVNALTNLFQFTNAVISIYPEIPDLKGQQQYFHGGPEGGIVIEDNEFETFDAPILYAKSVDGLVFRNNTIKLNTEYKPFHPNRNRFWLERVTNVTIAE

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 15-592aa

Sequence Info: Full Length of Mature Protein

MW: 69 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Alpha-galactosidase. Roves both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures .

Reference: Evolution of symbiotic bacteria in the distal human intestine.Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I.PLoS Biol. 5:1574-1586(2007)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures.

Involvement in disease:

Subcellular Location: Cell membrane, Lipid-anchor

Protein Families: Glycosyl hydrolase 110 family, B subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: A6KWM0

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A