RB1 (Ab-807) Antibody | CSB-PA445358

RB1 (Ab-807) Antibody | CSB-PA445358 | Cusabio

RB1 (Ab-807) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: RB1

Aliases: P105-RB; PP105; PP110; RB-1; RB1

Background: Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1) . It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2) . Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci) . SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3) . Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter

Roesch A, et al. (2005) Mod Pathol. 18 (4) : 565-572.
Chadee DN, et al. (2004) Nat Cell Biol. 6 (8) : 770-776.
Knudsen ES, et al. (1997) Mol Cell Biol. 17 (10) : 5771-5783.
Knudsen ES, et al. (1996) J Biol Chem. 271 (14) : 8313-8320.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P06400

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb.

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology