PTBP3 Antibody | CSB-PA020060NA01HU

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SKU:
CSB-PA020060NA01HU
Availability:
3 to 7 Working Days
  • PTBP3 Antibody
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate<br />
 All lanes: PTBP3 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 60, 57, 61, 50 kDa<br />
 Observed band size: 60 kDa<br />
  • IHC image of CSB-PA020060NA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunoprecipitating PTBP3 in A549 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA020060NA01HU in A549 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) <br />
 Lane 2: CSB-PA020060NA01HU (5µg) + A549 whole cell lysate (0.5mg) <br />
 Lane 3: A549 whole cell lysate (20µg) <br />
€315.80 - €488.70

Description

PTBP3 Antibody | CSB-PA020060NA01HU | Cusabio

PTBP3 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: PTBP3

Aliases: Polypyrimidine tract-binding protein 3 (Regulator of differentiation 1) (Rod1), PTBP3, ROD1

Background: RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly (G) and poly (U) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O95758

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence from Human Polypyrimidine tract-binding protein 3 protein (37-55AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IP

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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