Polyclonal Antibodies

Polyclonal Antibodies

Polyclonal Antibodies

Production of Polyclonal Antibodies Protocol:

1. Antigen Selection:

  • Choose the antigen against which you want to generate antibodies. It can be a protein, peptide, or other molecules. Order 30 amino acids as standard rule.

2. Animal Immunization:

  • Select a rabbit, goat, hamster, donkey, horse or sheep.
  • Inject the animal with the chosen antigen at regular intervals to stimulate the immune response.
  • day 1, 2, 11, 21, 31 bleed 51 booster, 61 2nd bleed
  • Bleed no more than 50 ml and give water to the rabbit after each bleed.
  • A cut in the ear with an old fashion razor blade is the easiest.
  • Put the rabbit at least 24 hours in a temperature above 15 C. In cold circomstances the bleed might be difficult
  • Use 10 tubes of 5 ml and dont mix the heamolised ones intp the pool.
  • The heamolised are due to a hair or a cascade of factros impossible to foresee, the rose bleeds are working fine too but its advised to not mix them with the yellow clear serum samples.

3. Booster Injections:

  • Administer booster injections to enhance the immune response and increase antibody production. Boosters are typically given at 10 days intervals after the initial 2 injections.

4. Blood Collection:

  • Monitor the immune response by taking small blood samples at regular intervals. Check for the presence of antibodies against the target antigen.

5. Serum Separation:

  • Once a sufficient antibody titer is reached, collect a larger blood sample, and separate the serum by coagulation 35 minutes and centrifugation from the blood cells. The serum contains the polyclonal antibodies.

6. Antibody Purification:

  • Purify the antibodies from the serum to isolate the immunoglobulin fraction. Common methods include protein A/G affinity chromatography or antigen affinity purification.
  • Crude srum works best because the best will stick to the antigen and wont let off even with guanidine

7. Characterization:

  • Characterize the purified antibodies for specificity, affinity, and concentration. Techniques such as ELISA or Western blotting can be used for this purpose. Dot blot is easiest.

8. Storage:

  • Store the purified polyclonal antibodies at -20 C to maintain stability and functionality.

 

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    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • IGF1 Antibody

    IGF1 Antibody | CSB-PA011086KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    IGF1 Antibody | CSB-PA011086KA01HU | CusabioIGF1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: IGF1Aliases:...
    SKU:
    CSB-PA011086KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • PATE3 Antibody

    PATE3 Antibody | CSB-PA017481KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    PATE3 Antibody | CSB-PA017481KA01HU | CusabioPATE3 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: PATE3Aliases:...
    SKU:
    CSB-PA017481KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • RAPGEF3 Antibody

    RAPGEF3 Antibody | CSB-PA019331KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    RAPGEF3 Antibody | CSB-PA019331KA01HU | CusabioRAPGEF3 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names:...
    SKU:
    CSB-PA019331KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • ABCC4 Antibody

    ABCC4 Antibody | CSB-PA001063KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    ABCC4 Antibody | CSB-PA001063KA01HU | CusabioABCC4 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: ABCC4Aliases:...
    SKU:
    CSB-PA001063KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • DLGAP5 Antibody

    DLGAP5 Antibody | CSB-PA006944KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    DLGAP5 Antibody | CSB-PA006944KA01HU | CusabioDLGAP5 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: DLGAP5Aliases:...
    SKU:
    CSB-PA006944KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • CLDN1 Antibody

    CLDN1 Antibody | CSB-PA005490KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    CLDN1 Antibody | CSB-PA005490KA01HU | CusabioCLDN1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: CLDN1Aliases:...
    SKU:
    CSB-PA005490KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • VDR Antibody

    VDR Antibody | CSB-PA025832KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    VDR Antibody | CSB-PA025832KA01HU | CusabioVDR Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: VDRAliases: VDR, VDR;...
    SKU:
    CSB-PA025832KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • RPS6KB1 Antibody

    RPS6KB1 Antibody | CSB-PA020470KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    RPS6KB1 Antibody | CSB-PA020470KA01HU | CusabioRPS6KB1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names:...
    SKU:
    CSB-PA020470KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • MAP2K7 Antibody

    MAP2K7 Antibody | CSB-PA013416KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    MAP2K7 Antibody | CSB-PA013416KA01HU | CusabioMAP2K7 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: MAP2K7Aliases:...
    SKU:
    CSB-PA013416KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • PPP2R2A Antibody

    PPP2R2A Antibody | CSB-PA018564KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    PPP2R2A Antibody | CSB-PA018564KA01HU | CusabioPPP2R2A Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names:...
    SKU:
    CSB-PA018564KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • PPP1CA Antibody

    PPP1CA Antibody | CSB-PA018503KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    PPP1CA Antibody | CSB-PA018503KA01HU | CusabioPPP1CA Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: PPP1CAAliases:...
    SKU:
    CSB-PA018503KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • PLCG2 Antibody

    PLCG2 Antibody | CSB-PA018135KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    PLCG2 Antibody | CSB-PA018135KA01HU | CusabioPLCG2 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: PLCG2Aliases:...
    SKU:
    CSB-PA018135KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • KRT17 Antibody

    KRT17 Antibody | CSB-PA012516KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    KRT17 Antibody | CSB-PA012516KA01HU | CusabioKRT17 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: KRT17Aliases:...
    SKU:
    CSB-PA012516KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • KCNQ1 Antibody

    KCNQ1 Antibody | CSB-PA012087KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    KCNQ1 Antibody | CSB-PA012087KA01HU | CusabioKCNQ1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: KCNQ1Aliases:...
    SKU:
    CSB-PA012087KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • ITGB2 Antibody

    ITGB2 Antibody | CSB-PA011884KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    ITGB2 Antibody | CSB-PA011884KA01HU | CusabioITGB2 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: ITGB2Aliases:...
    SKU:
    CSB-PA011884KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • IL10 Antibody

    IL10 Antibody | CSB-PA011580KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    IL10 Antibody | CSB-PA011580KA01HU | CusabioIL10 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: IL10Aliases: IL10;...
    SKU:
    CSB-PA011580KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • HCLS1 Antibody

    HCLS1 Antibody | CSB-PA010215KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    HCLS1 Antibody | CSB-PA010215KA01HU | CusabioHCLS1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: HCLS1Aliases:...
    SKU:
    CSB-PA010215KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • NR3C1 Antibody

    NR3C1 Antibody | CSB-PA016059KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    NR3C1 Antibody | CSB-PA016059KA01HU | CusabioNR3C1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: NR3C1Aliases:...
    SKU:
    CSB-PA016059KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • MAX Antibody

    MAX Antibody | CSB-PA013527KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    MAX Antibody | CSB-PA013527KA01HU | CusabioMAX Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: MAXAliases: MAX;...
    SKU:
    CSB-PA013527KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • Scn4b Antibody

    Scn4b Antibody | CSB-PA020842KA01MO

    Cusabio Polyclonal Antibodies

    €617.40
    Scn4b Antibody | CSB-PA020842KA01MO | CusabioScn4b Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: Scn4bAliases:...
    SKU:
    CSB-PA020842KA01MO
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • SPDYA Antibody

    SPDYA Antibody | CSB-PA022519KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    SPDYA Antibody | CSB-PA022519KA01HU | CusabioSPDYA Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: SPDYAAliases:...
    SKU:
    CSB-PA022519KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • KBTBD3 Antibody

    KBTBD3 Antibody | CSB-PA011998KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    KBTBD3 Antibody | CSB-PA011998KA01HU | CusabioKBTBD3 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: KBTBD3Aliases:...
    SKU:
    CSB-PA011998KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • ELSPBP1 Antibody

    ELSPBP1 Antibody | CSB-PA007629KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    ELSPBP1 Antibody | CSB-PA007629KA01HU | CusabioELSPBP1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names:...
    SKU:
    CSB-PA007629KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • Piwil1 Antibody

    Piwil1 Antibody | CSB-PA018045KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    Piwil1 Antibody | CSB-PA018045KA01HU | CusabioPiwil1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: Piwil1Aliases:...
    SKU:
    CSB-PA018045KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • EPPIN Antibody

    EPPIN Antibody | CSB-PA022583KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    EPPIN Antibody | CSB-PA022583KA01HU | CusabioEPPIN Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: EPPINAliases:...
    SKU:
    CSB-PA022583KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • SCCPDH Antibody

    SCCPDH Antibody | CSB-PA020801KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    SCCPDH Antibody | CSB-PA020801KA01HU | CusabioSCCPDH Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: SCCPDHAliases:...
    SKU:
    CSB-PA020801KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • LETMD1 Antibody

    LETMD1 Antibody | CSB-PA012879KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    LETMD1 Antibody | CSB-PA012879KA01HU | CusabioLETMD1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: LETMD1Aliases:...
    SKU:
    CSB-PA012879KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • REG3G Antibody

    REG3G Antibody | CSB-PA019549KA01MO

    Cusabio Polyclonal Antibodies

    €617.40
    REG3G Antibody | CSB-PA019549KA01MO | CusabioREG3G Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: Reg3gAliases:...
    SKU:
    CSB-PA019549KA01MO
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • GAS2L1 Antibody

    GAS2L1 Antibody | CSB-PA009266KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    GAS2L1 Antibody | CSB-PA009266KA01HU | CusabioGAS2L1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: GAS2L1Aliases:...
    SKU:
    CSB-PA009266KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • NDRG1 Antibody

    NDRG1 Antibody | CSB-PA015610KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    NDRG1 Antibody | CSB-PA015610KA01HU | CusabioNDRG1 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: NDRG1Aliases:...
    SKU:
    CSB-PA015610KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • NUMBL Antibody

    NUMBL Antibody | CSB-PA016187KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    NUMBL Antibody | CSB-PA016187KA01HU | CusabioNUMBL Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: NUMBLAliases:...
    SKU:
    CSB-PA016187KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • XRCC3 Antibody

    XRCC3 Antibody | CSB-PA026231KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    XRCC3 Antibody | CSB-PA026231KA01HU | CusabioXRCC3 Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: XRCC3Aliases:...
    SKU:
    CSB-PA026231KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)
    €617.40
    Qty in Cart: 0
    Quantity:
    Price:
    €617.40
    Subtotal:
  • WNT5A Antibody

    WNT5A Antibody | CSB-PA026138KA01HU

    Cusabio Polyclonal Antibodies

    €617.40
    WNT5A Antibody | CSB-PA026138KA01HU | CusabioWNT5A Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send quotation to info@gentaur.com.Product Type: Polyclonal AntibodyTarget Names: WNT5AAliases:...
    SKU:
    CSB-PA026138KA01HU
    Availability:
    3 to 7 Working Days
    Size:
    100ul (100ug)