POLDIP3 Antibody | CSB-PA871610LA01HU

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SKU:
CSB-PA871610LA01HU
Availability:
3 to 7 Working Days
  • POLDIP3 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate<br />
 All lanes: POLDIP3 antibody at 3.2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 47, 43 kDa<br />
 Observed band size: 43 kDa<br />
  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871610LA01HU at dilution of 1:100
  • Immunofluorescence staining of Hela cells with CSB-PA871610LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating POLDIP3 in Jurkat whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA871610LA01HU in Jurkat whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA871610LA01HU (8µg) + Jurkat whole cell lysate (500µg) <br />
 Lane 3: Jurkat whole cell lysate (10µg) <br />
€315.80 - €488.70

Description

POLDIP3 Antibody | CSB-PA871610LA01HU | Cusabio

POLDIP3 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: POLDIP3

Aliases: Polymerase delta-interacting protein 3 (46 kDa DNA polymerase delta interaction protein) (p46) (S6K1 Aly/REF-like target) (SKAR), POLDIP3, KIAA1649 PDIP46

Background: Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by assoociation with the TREX complex.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q9BY77

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Polymerase delta-interacting protein 3 protein (100-221AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:20-1:200

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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