Phospho-PTEN (Ser380/Thr382/Thr383) Antibody | CSB-PA909192

Phospho-PTEN (Ser380/Thr382/Thr383) Antibody | CSB-PA909192 | Cusabio

Phospho-PTEN (Ser380/Thr382/Thr383) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: PTEN

Aliases: MMAC1; Mutated in multiple advanced cancers 1; Protein-tyrosine phosphatase PTEN; TEP1;

Background:

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3, 4, 5-trisphosphate, phosphatidylinositol 3, 4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1, 3, 4, 5-tetrakisphosphate with order of substrate preference in vitro PtdIns (3, 4, 5) P3 > PtdIns (3, 4) P2 > PtdIns3P > Ins (1, 3, 4, 5) P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

Al-Khouri AM, et al. (2005) J Biol Chem. 280 (42) :35195-35202.
Torres J, et al. (2001) J Biol Chem. 276 (2) : 993-998.
Vazquez F, et al. (2000) Mol Cell Biol. 20 (14) : 5010-5018.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P60484

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S (p) -D-T (p) -T (p) -D-S) derived from Human PTEN.

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4: IF:1:100-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology;Metabolism;Signal transduction