Phospho-PAK1/PAK2/PAK3 (Thr423/Thr402/Thr421) Antibody | CSB-PA069102

Phospho-PAK1/PAK2/PAK3 (Thr423/Thr402/Thr421) Antibody | CSB-PA069102 | Cusabio

Phospho-PAK1/PAK2/PAK3 (Thr423/Thr402/Thr421) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: PAK1/PAK2/PAK3

Aliases: p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK;

Background:

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695.
Jung JH, et al. (2005) J Biol Chem; 280:40025-40031.
Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189
Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q13153/Q13177/O75914

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T (p) -M-V) derived from Human PAK1/PAK2/PAK3.

Immunogen Species: Human

Applications: ELISA, IHC, IF

Tested Applications: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: IHC:1:50-1:100

Dilution Ratio3: IF:1:100-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cancer;Cell biology;Signal transduction