Phospho-MAP2K3 (Thr222) Antibody | CSB-PA597904

Phospho-MAP2K3 (Thr222) Antibody | CSB-PA597904 | Cusabio

Phospho-MAP2K3 (Thr222) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: MAP2K3

Aliases: MAP2K3; MAPKK 3; MEK3; MP2K3; PRKMK3

Background:

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.

Derijard B., Science 267:682-685 (1995) .
Moriguchi T., J. Biol. Chem. 271:26981-26988 (1996) .
The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P46734

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Peptide sequence around phosphorylation site of threonine 222 (A-K-T (p) -M-D) derived from Human MAP2K3 .

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Immunology;Signal transduction