Cusabio Polyclonal Antibodies
Phospho-FOXO1 (Ser256) Antibody | CSB-PA589285
- SKU:
- CSB-PA589285
- Availability:
- 3 to 7 Working Days
- Size:
- 100ul
Description
Phospho-FOXO1 (Ser256) Antibody | CSB-PA589285 | Cusabio
Phospho-FOXO1 (Ser256) Antibody is Available at Gentaur Genprice with the fastest delivery.
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Product Type: Polyclonal Antibody
Target Names: FOXO1
Aliases: FKHR; FOXO1;
Background:
Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.
Isotype: IgG
Conjugate: Non-conjugated
Clonality: Polyclonal
Uniport ID: Q12778
Host Species: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Peptide sequence around phosphorylation site of serine 256 (A-A-S (p) -M-D) derived from Human FKHR.
Immunogen Species: Human
Applications: ELISA, WB, IHC, IF
Tested Applications: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200
Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Dilution Ratio1: ELISA:1:2000-1:10000
Dilution Ratio2: WB:1:500-1:1000
Dilution Ratio3: IHC:1:50-1:100
Dilution Ratio4: IF:1:100-1:200
Dilution Ratio5:
Dilution Ratio6:
Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form: liquid
Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Initial Research Areas: Signal Transduction
Research Areas: Epigenetics & Nuclear Signaling;Neuroscience;Cancer;Metabolism;Signal transduction