Phospho-EPHA2/EPHA3/EPHA4 (Tyr588/596) Antibody | CSB-PA438798

Phospho-EPHA2/EPHA3/EPHA4 (Tyr588/596) Antibody | CSB-PA438798 | Cusabio

Phospho-EPHA2/EPHA3/EPHA4 (Tyr588/596) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: EPHA2/EPHA3/EPHA4

Aliases: ECK; MPK-5; SEK2; kinase EphA2;

Background:

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

Lindberg R.A., Mol. Cell. Biol. 10:6316-6324 (1990) .
Zhang Y., Mol. Cell. Proteomics 4:1240-1250 (2005) .
Zhuang G., J. Biol. Chem. 282:2683-2694 (2007)

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P29317/P29320/P54764

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y (p) -V-R) / (R-T-Y (p) -V-D) derived from Human EPHA2/3/4.

Immunogen Species: Human

Applications: ELISA, WB, IF

Tested Applications: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IF:1:100-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cardiovascular

Research Areas: Neuroscience;Cardiovascular