Phospho-CDC25B (Ser353) Antibody | CSB-PA570157

Phospho-CDC25B (Ser353) Antibody | CSB-PA570157 | Cusabio

Phospho-CDC25B (Ser353) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: CDC25B

Aliases: CDC25M2; MPIP2; Dual specificity phosphatase Cdc25B; CDC25HU2;

Background:

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Reboutier D, et al. (2012) J Cell Biol 197, 19-26
Lobjois V, et al. (2011) Biochem Biophys Res Commun 410, 87-90
Feng C, et al. (2007) Biol Reprod 77, 560-8

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P30305

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around phosphorylation site of serine 353 (R-R-S (p) -V-T) derived from Human CDC25B .

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:1000

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Epigenetics & Nuclear Signaling;Cancer;Cell biology