Phospho-BLM (Thr99) Antibody | CSB-PA188391

Phospho-BLM (Thr99) Antibody | CSB-PA188391 | Cusabio

Phospho-BLM (Thr99) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: BLM

Aliases: RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3

Background:

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE) .

Ellis N.A., Cell 83:655-666 (1995) .
Karow J.K., J. Biol. Chem. 272:30611-30614 (1997) .
Pichierri P., EMBO J. 23:3154-3163 (2004) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P54132

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around phosphorylation site of threonine 99 (Q-E-T (p) -Q-R) derived from Human Bloom Syndrome.

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:1000

Dilution Ratio3: IHC:1:50-1:100

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling